Listeria monocytogenes Detection Kit
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Description
Rapid and specific detection of Listeria monocytogenes in foods The SAS™ Listeria monocytogenes Detection Kit uses the Listeria monocytogenes invasion gene (iap) as a target sequence. By using four primers to recognize six distinct regions in the gene, this test is highly specific. Due to the high amplification efficiency of isothermal amplification technology (Loopamp®), the results are achieved within one hour. Including pre-enrichment culture and DNA purification, testing of Listeria monocytogenes in foods has a turnaround time of 24 hours using this test. No further enrichment culture is needed. Amplification and detection in one single-tube with real-time results. With Realtime Turbidimeters, amplification and real-time detection are achieved in one step. Since electrophoresis is not required, the entire process is completed within one reaction tube, which greatly reduces the possibility of cross-contamination frequently associated with nucleic acid amplification technologies. Alternative method of detection Because this is an isothermal reaction, the testing can also be performed in a water bath or a heat block set at 65°C. The endpoint results can be visualized by adding Fluorescent Detection Reagent (sold separately) to the initial master mix.
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Listeria monocytogenes Detection Kit
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